Anti-FOXA1抗体[3A8] (ab117857)


  • 产品名称Anti-FOXA1抗体[3A8]
    参阅全部 FOXA1 一抗
  • 描述
    小鼠单克隆抗体[3A8] to FOXA1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Dog, Human
  • 免疫原

    Recombinant protein expressed in E.coli, corresponding to amino acids 1-472 of Human FOXA1.

  • 阳性对照
    • WB: HEK293T transfected lysate; extract from MDCK lysate cell line ICC/IF: COS7 transfected cells
  • 常规说明Dilute in PBS (pH 7.3) before use.



Our Abpromise guarantee covers the use of ab117857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 49 kDa.
ICC/IF Use at an assay dependent dilution.


  • 功能Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • 组织特异性Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • forkhead box A1 antibody
    • Forkhead box protein A1 antibody
    • FOX A1 antibody
    • FOXA1 antibody
    • FOXA1_HUMAN antibody
    • hepatocyte nuclear factor 3 alpha antibody
    • Hepatocyte nuclear factor 3-alpha antibody
    • HNF 3A antibody
    • HNF-3-alpha antibody
    • HNF-3A antibody
    • HNF3A antibody
    • MGC33105 antibody
    • TCF 3A antibody
    • TCF-3A antibody
    • TCF3A antibody
    • Transcription factor 3A antibody
    see all

Anti-FOXA1 antibody [3A8] 图像

  • All lanes : Anti-FOXA1 antibody [3A8] (ab117857) at 1/1000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY FOXA1 cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 49 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206045)
  • All lanes : Anti-FOXA1 antibody [3A8] (ab117857) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : HT29 lysate
    Lane 4 : A549 lysate
    Lane 5 : COS7 lysate
    Lane 6 : Jurkat lysate
    Lane 7 : MDCK lysate
    Lane 8 : PC12 lysate
    Lane 9 : MCF7 lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 49 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206045)
  • Immunofluorescent staining of COS7 cells transiently transfected with pCMV6-ENTRY FOXA1, using ab117857.

Anti-FOXA1 antibody [3A8] (ab117857)参考文献

ab117857 has not yet been referenced specifically in any publications.

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