概述

  • 产品名称Anti-Fox2 / RBM9抗体
    参阅全部 Fox2 / RBM9 一抗
  • 描述
    小鼠单克隆抗体to Fox2 / RBM9
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cyt, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: MEKKKMVTQG NQEPTTTPDA MVQPFTTIPF PPPPQNGIPT EYGVPHTQDY AGQTGEHNLT LYGSTQAHGE QSSNSPSTQN GSLTTEGGAQ TDGQQSQTQS , corresponding to amino acids 1-100 of Human Fox2/ RBM9

性能

应用

Our Abpromise guarantee covers the use of ab57154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HRNBP2 antibody
    • Fox 1 homologue antibody
    • Fox-1 homolog B antibody
    • FOX2 antibody
    • FXH antibody
    • Hexaribonucleotide-binding protein 2 antibody
    • HNRBP2 antibody
    • RBFOX2 antibody
    • Repressor of tamoxifen transcriptional activity antibody
    • RFOX2_HUMAN antibody
    • RNA binding motif protein 9 antibody
    • RNA binding protein 9 antibody
    • RNA binding protein fox-1 homolog 2 antibody
    • RNA-binding motif protein 9 antibody
    • RNA-binding protein 9 antibody
    • RTA antibody
    see all

Anti-Fox2 / RBM9 antibody 图像

  • Fox2 / RBM9 antibody (ab57154) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.


  • Predicted band size : 41 kDa
    Fox2 / RBM9 antibody (ab57154) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • ICC/IF image of ab57154 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57154, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab57154 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57154, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Fox2 / RBM9 antibody (ab57154)参考文献

This product has been referenced in:
  • Verma SK  et al. Reactivation of fetal splicing programs in diabetic hearts is mediated by protein kinase C signaling. J Biol Chem 288:35372-86 (2013). WB ; Rat . Read more (PubMed: 24151077) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (fetal lung)
Loading amount 20 µg
Specification fetal lung
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain coronal)
Specification Brain coronal
Fixative Paraformaldehyde
Permeabilization Yes - PBS + 0.1% Tween20
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Melanie White

Verified customer

提交于 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"