Anti-Fos B抗体[83B1138] (ab11959)

概述

  • 产品名称
    Anti-Fos B抗体[83B1138]
    参阅全部 Fos B 一抗
  • 描述
    小鼠单克隆抗体[83B1138] to Fos B
  • 经测试应用
    适用于: Flow Cyt, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    This antibody was raised against a synthetic peptide corresponding to human Fos B.

  • 阳性对照
    • HeLa whole-cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab11959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FoFr 1/500 - 1/5000. According to the materials and methods by Carlsson et al. (2005) Brain 128:559-569 "the midbrain was fixed in 4% paraformaldehyde (PFA), in 0.1 M phosphate buffer, pH 7.4, for 24h and dehydrated in 25% sucrose and cut on a freezing microtome" therefore fixation was by immersion, not perfusion.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).

靶标

图片



  • Predicted band size : 40.6 kDa
    ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.
  • Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.

    See Abreview

  • ab11959 Fos B antibody (1/1000) immunostaining for Fos B in rat brain cortex. Staining was also detected in the striatum as expected (not shown). Rats were treated with high dose amphetamine (5mg/kg) 30 min prior to transcardial perfusion to induce Fos B expression. The staining procedure was perfomed according to the TH staining in Carlsson et al. (2005) Brain 128:559-569 with the exception that the quenching step was excluded from the staining.

文献

This product has been referenced in:
  • Buenrostro-Jáuregui M  et al. Changes in CREB and deltaFosB are associated with the behavioural sensitization induced by methylenedioxypyrovalerone. J Psychopharmacol 30:707-12 (2016). Read more (PubMed: 27147595) »
  • Chen T  et al. Postsynaptic potentiation of corticospinal projecting neurons in the anterior cingulate cortex after nerve injury. Mol Pain 10:33 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24890933) »

See all 6 Publications for this product

客户评价及客户问答

Thank you for your enquiry. With this antibody we usually detect a second band of approximately 34 kDa which is delta-FosB. I hope this information will be useful to you. Should you require any other information, please do not hesitate to contact...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册