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Our Abpromise guarantee covers the use of ab11959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-FoFr||1/500 - 1/5000. According to the materials and methods by Carlsson et al. (2005) Brain 128:559-569 "the midbrain was fixed in 4% paraformaldehyde (PFA), in 0.1 M phosphate buffer, pH 7.4, for 24h and dehydrated in 25% sucrose and cut on a freezing microtome" therefore fixation was by immersion, not perfusion.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).|
ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.
ab11959 Fos B antibody (1/1000) immunostaining for Fos B in rat brain cortex. Staining was also detected in the striatum as expected (not shown). Rats were treated with high dose amphetamine (5mg/kg) 30 min prior to transcardial perfusion to induce Fos B expression. The staining procedure was perfomed according to the TH staining in Carlsson et al. (2005) Brain 128:559-569 with the exception that the quenching step was excluded from the staining.
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