Anti-Fos B抗体[83B1138] (ab11959)


  • 产品名称Anti-Fos B抗体[83B1138]
    参阅全部 Fos B 一抗
  • 描述
    小鼠单克隆抗体[83B1138] to Fos B
  • 经测试应用适用于: Flow Cyt, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    This antibody was raised against a synthetic peptide corresponding to human Fos B.

  • 阳性对照
    • HeLa whole-cell extract.



Our Abpromise guarantee covers the use of ab11959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-FoFr 1/500 - 1/5000. According to the materials and methods by Carlsson et al. (2005) Brain 128:559-569 "the midbrain was fixed in 4% paraformaldehyde (PFA), in 0.1 M phosphate buffer, pH 7.4, for 24h and dehydrated in 25% sucrose and cut on a freezing microtome" therefore fixation was by immersion, not perfusion.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 40.6 kDa).


Anti-Fos B antibody [83B1138] 图像

  • Predicted band size : 40.6 kDa
    ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB. ab11959 staining Human Fos B (upper band) in HeLa whole cell lysate by Western blot (ECL). The lower band is thought to be an isoform of FosB.
  • Overlay histogram showing HeLa cells stained with ab11959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11959, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab11959 Fos B antibody immunostaining (red) in mouse brain cortex showing the striatum. Tissue was fixed with paraformaldehyde, before incubation with the antibody (1/3000) for 18 hours at 25ºC. A donkey anti-mouse Alexa Fluor® 568 conjugated secondary was used at 1/1000.

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  • ab11959 Fos B antibody (1/1000) immunostaining for Fos B in rat brain cortex. Staining was also detected in the striatum as expected (not shown). Rats were treated with high dose amphetamine (5mg/kg) 30 min prior to transcardial perfusion to induce Fos B expression. The staining procedure was perfomed according to the TH staining in Carlsson et al. (2005) Brain 128:559-569 with the exception that the quenching step was excluded from the staining.

Anti-Fos B antibody [83B1138] (ab11959)参考文献

This product has been referenced in:
  • Buenrostro-Jáuregui M  et al. Changes in CREB and deltaFosB are associated with the behavioural sensitization induced by methylenedioxypyrovalerone. J Psychopharmacol 30:707-12 (2016). Read more (PubMed: 27147595) »
  • Chen T  et al. Postsynaptic potentiation of corticospinal projecting neurons in the anterior cingulate cortex after nerve injury. Mol Pain 10:33 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24890933) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - TritonX-100
Specification Brain
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Jun 15 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde

Dr. Karine Thibault

Verified customer

提交于 Aug 19 2014

Thank you for your enquiry. With this antibody we usually detect a second band of approximately 34 kDa which is delta-FosB. I hope this information will be useful to you. Should you require any other information, please do not hesitate to contact...

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