概述

性能

应用

Our Abpromise guarantee covers the use of ab58920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Follistatin antibody 图像

  • Lane 1 : Anti-Follistatin antibody (ab58920) at 1 µg/ml
    Lane 2 : Anti-Follistatin antibody (ab58920) at 2.5 µg/ml
    Lane 3 : Anti-Follistatin antibody (ab58920) at 5 µg/ml

    Lane 1 : Cell lysate prepared from human Jurkat cells
    Lane 2 : Cell lysate prepared from human Jurkat cells
    Lane 3 : Cell lysate prepared from human Jurkat cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa

实验方案

Anti-Follistatin antibody (ab58920)参考文献

This product has been referenced in:
  • Jones AE  et al. Wnt/ß-catenin controls follistatin signalling to regulate satellite cell myogenic potential. Skelet Muscle 5:14 (2015). Read more (PubMed: 25949788) »
  • Gao MH  et al. beta(1)-Adrenergic receptor vs adenylyl cyclase 6 expression in cardiac myocytes: differences in transgene localization and intracellular signaling. Cell Signal 22:584-9 (2010). Read more (PubMed: 19932173) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine Gels)
Loading amount
70 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. John Odhiambo

Verified customer

提交于 Oct 22 2015

DISCOUNT CODE:xxxxxxx
Expiration date: 20/03/2013
Value: CAD356.00
I am very pleased to offer you to test ab58920 in zebrafish. Because the only tested application for this product is WB it would be imprtant to tests the antibody on zebraf...

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Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab58920 has been added to yo...

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Thank you for your message and for providing this further information.

I can confirm, as previously discussed, that the recommended concentration for Western blot using this antibody is 2.5 µg/ml (1:400) as stated on the datasheet. This is a...

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Sorry for the misunderstanding. Both antibodies have been developed to recognise specifically the follistatin. I would say that both products are equally specific to the target. The polyclonal antibody was raised against a 49 amino acid l...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic mouse head E16)
Specification
Embryonic mouse head E16
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Gaskon Ibarretxe

Verified customer

提交于 Feb 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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