Anti-Folate Binding Protein抗体[LK26] (ab3361)

概述

  • 产品名称
    Anti-Folate Binding Protein抗体[LK26]
    参阅全部 Folate Binding Protein 一抗
  • 描述
    小鼠单克隆抗体[LK26] to Folate Binding Protein
  • 经测试应用
    适用于: IHC-Fr, IP, Flow Cyt, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Gestational choriocarcinoma cell line, Lu-75.

  • 阳性对照
    • Frozen Placenta or Ovarian Carcinoma (Negative control: Mouse serum or IgG1 isotype-matched negative control antibody, diluted to the same concentration as the primary antibody, should also be included in each run.)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    LK26
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/40.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 19567824
ICC/IF Use at an assay dependent concentration. PubMed: 19636639
WB 1/500. PubMed: 18553957

靶标

  • 功能
    Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • 组织特异性
    Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
  • 疾病相关
    Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
  • 序列相似性
    Belongs to the folate receptor family.
  • 翻译后修饰
    Eight disulfide bonds are present.
    The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
  • 细胞定位
    Cell membrane. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • adult antibody
    • Adult folate binding protein antibody
    • Adult folate-binding protein antibody
    • FBP antibody
    • Folate Receptor 1 Adult antibody
    • Folate receptor 1 antibody
    • Folate Receptor 1 Precursor antibody
    • Folate receptor adult antibody
    • Folate receptor alpha antibody
    • Folate receptor antibody
    • FOLR antibody
    • FOLR1 antibody
    • FOLR1_HUMAN antibody
    • FR alpha antibody
    • FR-alpha antibody
    • FRalpha antibody
    • KB cells FBP antibody
    • MOV18 antibody
    • Ovarian cancer associated antigen antibody
    • Ovarian tumor associated antigen antibody
    • Ovarian tumor associated antigen MOv18 antibody
    • Ovarian tumor-associated antigen MOv18 antibody
    see all

图片

  • ab3361 staining frozen placenta in IHC-F.

文献

This product has been referenced in:
  • Xie M  et al. Expression of folate receptors in nasopharyngeal and laryngeal carcinoma and folate receptor-mediated endocytosis by molecular targeted nanomedicine. Int J Nanomedicine 8:2443-51 (2013). Human . Read more (PubMed: 23874095) »
  • Roger E  et al. Folic Acid Functionalized Nanoparticles for Enhanced Oral Drug Delivery. Mol Pharm : (2012). Flow Cyt . Read more (PubMed: 22670575) »

See all 6 Publications for this product

客户评价及客户问答

It will depend on how you want to use the antibody and how much is required for each test . I updated the ab2107 datasheet to show suggested dilution ranges for the applications that are listed on the datasheet.

WB: 1/500 - 1/5000.
IP: ...

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Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement with ab125030, the order number is *******.

To check the status of the order please contact our Customer Service...

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Gracias por tu respuesta.

Si te parece puedo enviaros para que probéis un anticuerpo diferente contra la misma proteína.

Echarle un vistazo a los otros productos que tenemos disponibles contra Folate Binding Protein va...

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Muchas gracias por mandarnos el cuestionario relleno.

Para intentar optimizar los resultados del anticuerpo hay una serie de sugerencias que me gustaría hacer. De cualquier manera, al estar al anticuerpo bajo garantía en caso de...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP-1)
Specification
THP-1
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2008

Thank you for your enquiry. Ab3361 was generated using gestational choriocarcinoma cell line, Lu-75, as the immunogen. However, we have not conducted testing to determine whether the antibody is specific for one or the other subtype of the receptor. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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