Anti-Folate Binding Protein抗体(ab67422)

概述

  • 产品名称Anti-Folate Binding Protein抗体
    参阅全部 Folate Binding Protein 一抗
  • 描述
    兔多克隆抗体to Folate Binding Protein
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Folate Binding Protein.

    (Peptide available as ab87124.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Cerebellum Tissue Lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). Please note, we have been advised by some customers that ab67422 is unable to detect the human version of this protein in western blot. Please contact our scientific support services if you have any queries regarding this antibody.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells.
  • 组织特异性Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum.
  • 疾病相关Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function.
  • 序列相似性Belongs to the folate receptor family.
  • 翻译后修饰Eight disulfide bonds are present.
    The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
  • 细胞定位Cell membrane. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • adult antibody
    • Adult folate binding protein antibody
    • Adult folate-binding protein antibody
    • FBP antibody
    • Folate Receptor 1 Adult antibody
    • Folate receptor 1 antibody
    • Folate Receptor 1 Precursor antibody
    • Folate receptor adult antibody
    • Folate receptor alpha antibody
    • Folate receptor antibody
    • FOLR antibody
    • FOLR1 antibody
    • FOLR1_HUMAN antibody
    • FR alpha antibody
    • FR-alpha antibody
    • FRalpha antibody
    • KB cells FBP antibody
    • MOV18 antibody
    • Ovarian cancer associated antigen antibody
    • Ovarian tumor associated antigen antibody
    • Ovarian tumor associated antigen MOv18 antibody
    • Ovarian tumor-associated antigen MOv18 antibody
    see all

Anti-Folate Binding Protein antibody 图像

  • Anti-Folate Binding Protein antibody (ab67422) at 1/1 dilution + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa (possible cleavage fragment),22 kDa (possible cleavage fragment).
  • IHC image of Folate Binding Protein staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67422, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab67422 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67422 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Folate Binding Protein antibody (ab67422)参考文献

ab67422 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and for sending the details of the customer's complaint.
I am sorry that this antibody has not given the expected results. We have actually received reports that this antibody does not work well in Western blot with hum...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney (untreated CD1 mouse))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6, 20 min, 98C
Permeabilization No
Specification Kidney (untreated CD1 mouse)
Blocking step Dako S2023 as blocking agent for 10 minute(s) · Concentration: 0µg/mL · Temperature: RT°C
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Oct 30 2015

Thank you for taking the time to complete our questionnaire and sending the images. I am sorry to hear you have had difficulty obtaining satisfactory results from our products.
Reviewing this case, I would like to offer some suggestions to help op...

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Thank you for contacting us and sending the image.
Without further details it is very difficult to say where these bands observed come from. I am very happy to help you, and for that, I would appreciate if you could please send me more protocol de...

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Thank you for your enquiry. I have also run a BLAST homology and the results is as below. gi|148225128|ref|NP_001086114.1| folate receptor 1 (adult) [Xenopus laevis] gi|49257600|gb|AAH74206.1| MGC82130 protein [Xenopus laevis] Length=243 ...

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