重组Anti-FNTB抗体[EPR4707] (ab109625)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4707] to FNTB
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FNTB抗体[EPR4707]
参阅全部 FNTB 一抗 -
描述
兔单克隆抗体[EPR4707] to FNTB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- JAR, A431, U87-MG and A375 cell lysates, Human breast tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4707 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109625于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding. -
序列相似性
Belongs to the protein prenyltransferase subunit beta family.
Contains 5 PFTB repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 2342 Human
- Omim: 134636 Human
- SwissProt: P49356 Human
- Unigene: 632345 Human
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别名
- CAAX farnesyltransferase subunit beta antibody
- EC 2.5.1.58 antibody
- EC=2.5.1.58 antibody
see all
图片
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All lanes : Anti-FNTB antibody [EPR4707] (ab109625) at 1/10000 dilution
Lane 1 : JAR cell lysate
Lane 2 : A431 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109625 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109625 被引用在 4 文献中.
- Hadfield JD et al. Cell Synchronization Techniques for Studying Mitosis. Methods Mol Biol 2579:73-86 (2022). PubMed: 36045199
- Pinner AL et al. Protein expression of prenyltransferase subunits in postmortem schizophrenia dorsolateral prefrontal cortex. Transl Psychiatry 10:3 (2020). PubMed: 32066669
- Makhov P et al. CRISPR/Cas9 genome-wide loss-of-function screening identifies druggable cellular factors involved in sunitinib resistance in renal cell carcinoma. Br J Cancer 123:1749-1756 (2020). PubMed: 32968206
- Liang J et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). PubMed: 29670091