概述

  • 产品名称Anti-FNDC3B抗体
    参阅全部 FNDC3B 一抗
  • 描述
    兔多克隆抗体to FNDC3B
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: SCTTVYNGYG KGHSGGSGGG GSGSGPGIKK TERRARSSPK SNDSDLQEYE , corresponding to internal sequence amino acids 217-266 of Human FNDC3B

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab69854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 133 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 相关性FNDC3B may be a positive regulator of adipogenesis.
  • 细胞定位Membrane; Single pass membrane protein
  • 数据库链接
  • 别名
    • DKFZp686D14170 antibody
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    • NS5ABP37 antibody
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    see all

Anti-FNDC3B antibody 图像

  • Anti-FNDC3B antibody (ab69854) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

Anti-FNDC3B antibody (ab69854)参考文献

ab69854 has not yet been referenced specifically in any publications.

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