概述

  • 产品名称
  • 描述
    兔多克隆抗体to FMN2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) corresponding to a region between residues 650-700 of human FMN2. (NP_064450.3).

  • 阳性对照
    • human brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明
    WB: Use at a concentration of 0.25 - 0.5 µg/ml. Detects bands of approximately 67 and 150 kDa (predicted molecular weight: 180 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (By similarity). Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Protects cells against apoptosis by protecting CDKN1A against degradation.
    • 组织特异性
      Expressed almost exclusively in the developing and mature central nervous system.
    • 序列相似性
      Belongs to the formin homology family. Cappuccino subfamily.
      Contains 1 FH1 (formin homology 1) domain.
      Contains 1 FH2 (formin homology 2) domain.
    • 细胞定位
      Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cytoplasm > cytosol. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm > cell cortex. Nucleus > nucleolus. Recruited to the membranes via its interaction with SPIRE1. Colocalizes with the actin cytoskeleton. Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FMN 2 antibody
      • Fmn2 antibody
      • FMN2_HUMAN antibody
      • Formin 2 antibody
      • Formin-2 antibody
      see all

    图片

    • All lanes : Anti-FMN2 antibody (ab72052) at 0.25 µg/ml

      Lane 1 : human brain lysate
      Lane 2 : human brain lysate with immunising peptide


      Predicted band size : 180 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

    文献

    This product has been referenced in:
    • Yu XJ  et al. The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics. Nat Commun 5:4887 (2014). Read more (PubMed: 25208553) »
    • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

    See all 2 Publications for this product

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