antigen sequence: SKDSTKVAGA KRKGVHCVMS LGVPGPATLA KALLQTHPEA QRAIEAAPQE PEQKRSRQDP GTDRTEDSGL AAGPPEAAGE NFA, corresponding to amino acids 48-130 of Human FLYWCH2.
- Human prostate tissue; FLYWCH2 over-expression HEK293T lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- FLYWCH family member 2 antibody
- Flywch2 antibody
- FWCH2_HUMAN antibody
- OTTHUMP00000159209 antibody
Immunocytochemistry/ Immunofluorescence - Anti-FLYWCH2 antibody (ab122712)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122712 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLYWCH2 antibody (ab122712)
ab122712, at 1/50 dilution, staining FLYWCH2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Western blot - Anti-FLYWCH2 antibody (ab122712)
All lanes : Anti-FLYWCH2 antibody (ab122712) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : FLYWCH2 over-expression HEK293T lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"