Fluoroshield封固剂With DAPI (20ml) (ab104139)

概述

  • 产品名称
    Fluoroshield封固剂With DAPI (20ml)
    参阅全部 Fluoroshield 试剂
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, IHC-Pmore details
  • 常规说明
    Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
    May encounter problems with FROZEN BRAIN OR OTHER FROZEN TISSUES WITH LOTS OF FAT.

    Procedure
    • Bring the vial to room temperature.
    • Rinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
    • Turn the vial upside down and open the dropper to remove any air bubbles.
    • Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.
    • Let stand at room temperature for about 5 minutes.
    • Apply cover slip carefully avoiding air bubbles.
    • The specimen is ready for visualization under a microscope.
    • One can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (ab104141). If a coverslip is not sealed air bubbles will appear in few days.
    • For storage it is recommended that the slide be stored in the dark at 2-8°C.

    Removal of Coverslip
    Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

性能

应用

Our Abpromise guarantee covers the use of ab104139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

图片

  • Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI (ab104139).

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文献

This product has been referenced in:
  • Telegina DV  et al. Contributions of age-related alterations of the retinal pigment epithelium and of glia to the AMD-like pathology in OXYS rats. Sci Rep 7:41533 (2017). ICC/IF ; Rat . Read more (PubMed: 28134357) »
  • Poulsen ET  et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci 10:59 (2017). ICC/IF . Read more (PubMed: 28360834) »

See all 35 Publications for this product

客户评价及客户问答

Best quality-price ratio

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've tried some others, but I'll definitively stick with this one. Nice stain and affordable price.
Username

Abcam user community

Verified customer

提交于 Aug 31 2017

Excellent and convenient

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I've used a number of various mounting media - SouthernBiotech, Invitrogen/Life Tech, Vectashield, and now this. This one is my favorite for several reasons: (1) Affordability, (2) Packaging, and (3) ease of use.
Username

Abcam user community

Verified customer

提交于 Jul 31 2017

Good Solution for fast Staining

Excellent Excellent 5/5 (Ease of Use)
Abreviews
It works as expected. Picture shows an example of DAPI positive nuclei.
Username

Abcam user community

Verified customer

提交于 Aug 19 2016

Works great!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This dye works great for frozen or paraffin embedded skin tissue in all our experiments.
Username

Ahmar Aziz

Verified customer

提交于 May 05 2016

Excellent results

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS and stained with undiluted Fluoroshield Mounting Medium with DAPI.
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Mar 09 2016

Fantastic!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I was worried about some of the reviews but the price made it an easy decision. I was pretty happy since it worked exactly as it should in resin sections and stained heterochromatic areas of the nucleus
Username

Sebastian Alvarado

Verified customer

提交于 Dec 08 2015

Thank you for your reply. While we do not generally give discounts for teaching institutions in particular, if you are starting a new lab, moving a lab, or a first-time grant recipient, you may qualify for our New Lab Discount Program:

http:/...

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Thank you for contacting us.


We do not currently sell DAPI that is not contained in mounting media, but the red fluorescent DRAQ5 dye (ab108410) can be used in a very similar fashion to produce nuclear staining on fixed tissue. Alternati...

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Thank you for contacting us.

Either of these antibodies would be suitable for your purposes. You could also use our beta Actin antibody ab6277, which has been validated for use on chicken and cow samples in IHC-Fr as well. Beta Actin is commo...

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Thank you for your phone call.

As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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