概述

  • 产品名称Anti-Fluorescein抗体(HRP)
    参阅全部 Fluorescein 一抗
  • 描述
    兔多克隆抗体to Fluorescein (HRP)
  • 偶联物HRP
  • 特异性Specific to Fluoresceine.
  • 经测试应用适用于: IP, IHC-P, WB, ELISAmore details
  • 种属反应性
    No reactivity with immunoglobulin of any species.
  • 免疫原

    Fluorescein coupled to KLH

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA
  • Concentration information loading...
  • 纯化说明Solid phase hapten antibody capture. Affinity purified and absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated substitution ratio: HRP:IgG is >1.5
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 19706527
IHC-P Use at an assay dependent concentration.
WB 1/20 - 1/200.
ELISA 1/200 - 1/1000.

靶标

  • 相关性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 别名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (HRP) 图像

  • Immunohistochemical analysis of chicken trachea tissue, infected with an avian influenza virus, staining Fluorescein with ab19492.
    Fluoroscein-labeled influenza viruses were incubated with tissue overnight at 4°C. Sections were incubated with primary antibody and the signal was amplified with a tyramide signal amplification system.

Anti-Fluorescein antibody (HRP) (ab19492)参考文献

This product has been referenced in:
  • Kraaij MD  et al. The NOX2-mediated ROS producing capacity of recipient cells is associated with reduced T cell infiltrate in an experimental model of chronic renal allograft inflammation. Transpl Immunol 30:65-70 (2014). Read more (PubMed: 24365514) »
  • Jourdain E  et al. The Pattern of Influenza Virus Attachment Varies among Wild Bird Species. PLoS One 6:e24155 (2011). IHC-P . Read more (PubMed: 21909418) »

See all 4 Publications for this product

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Without a direct comparison, it is a little difficult to choose between the two. The ab6656 datasheet also has a review of IHC with TSA amplification on frozen tissue, after applying the antibody at a dilution of...

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