Anti-Fluorescein抗体[BT.6A4] (Biotin) (ab6215)

概述

  • 产品名称
    Anti-Fluorescein抗体[BT.6A4] (Biotin)
    参阅全部 Fluorescein 一抗
  • 描述
    小鼠单克隆抗体[BT.6A4] to Fluorescein (Biotin)
  • 偶联物
    Biotin
  • 特异性
    The antibody reacts with free and bound fluorescein.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明
    Purified anti-fluorescein biotin conjugate.
  • 克隆
    单克隆
  • 克隆编号
    BT.6A4
  • 骨髓瘤
    unknown
  • 同种型
    IgM
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
In situ hybridization Use at an assay dependent dilution.

靶标

  • 相关性
    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 别名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody [BT.6A4] (Biotin) (ab6215)参考文献

ab6215 has not yet been referenced specifically in any publications.

Product Wall

You could detect the biotinylated antibody directly or use a secondary to the fluorescein antibody.

This antibody has not been tested in flow cytometry. However, in theory it should work in your application.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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