Alkaline Phosphatase Anti-Fluorescein抗体(ab6657)
Key features and details
- Alkaline Phosphatase Goat polyclonal to Fluorescein
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
概述
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产品名称
Alkaline Phosphatase Anti-Fluorescein抗体
参阅全部 Fluorescein 一抗 -
描述
Alkaline Phosphatase山羊多克隆抗体to Fluorescein -
宿主
Goat -
偶联物
Alkaline Phosphatase -
经测试应用
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
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常规说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot |
1/1000 - 1/5000.
|
|
ELISA |
1/1000 - 1/5000.
|
|
IHC-P |
1/1000 - 1/5000.
|
|
IHC-Fr |
1/1000 - 1/5000.
|
|
WB |
1/1000 - 1/5000.
|
说明 |
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Dot blot
1/1000 - 1/5000. |
ELISA
1/1000 - 1/5000. |
IHC-P
1/1000 - 1/5000. |
IHC-Fr
1/1000 - 1/5000. |
WB
1/1000 - 1/5000. |
靶标
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相关性
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
别名
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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