Anti-Fluorescein抗体(Alkaline Phosphatase) (ab6657)

概述

  • 产品名称Anti-Fluorescein抗体(Alkaline Phosphatase)
    参阅全部 Fluorescein 一抗
  • 描述
    山羊多克隆抗体to Fluorescein (Alkaline Phosphatase)
  • 偶联物Alkaline Phosphatase
  • 经测试应用适用于: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
  • 免疫原

    Fluorescein conjugated to Goat IgG

  • 常规说明

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/1000 - 1/5000.
ELISA 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
WB 1/1000 - 1/5000.

靶标

  • 相关性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 别名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (Alkaline Phosphatase) (ab6657)参考文献

ab6657 has not yet been referenced specifically in any publications.

Product Wall

Based on the Lot #6189 of ab6657, a working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product (assayed against 1.0 ug of Fluorescein conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"