概述

  • 产品名称Anti-Fluorescein抗体
    参阅全部 Fluorescein 一抗
  • 描述
    兔多克隆抗体to Fluorescein
  • 特异性Specific to Fluoresceine. No cross reactivity with immunoglobulin of any species.
  • 经测试应用适用于: ELISA, WBmore details
  • 免疫原

    Fluoresceine conjugated to KHL.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Immunoaffinity chromatography
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

靶标

  • 相关性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 别名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (ab19491)参考文献

This product has been referenced in:
  • Valentin-Hansen L  et al. Mapping Substance P Binding Sites on the Neurokinin-1 Receptor Using Genetic Incorporation of a Photoreactive Amino Acid. J Biol Chem 289:18045-18054 (2014). WB . Read more (PubMed: 24831006) »
  • Ishizuka A  et al. Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1. Genes Cells 19:704-21 (2014). Read more (PubMed: 25145264) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry.

I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in...

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The reactivity of ab19491 has not been tested against FAM, this polyclonal antibody has been tested and will react with DTAF ( a fluorescein derivative) in addition to FITC.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"