Anti-Flt3 ligand抗体[EP1140Y] (ab52648)


  • 产品名称Anti-Flt3 ligand抗体[EP1140Y]
    参阅全部 Flt3 ligand 一抗
  • 描述
    兔单克隆抗体[EP1140Y] to Flt3 ligand
  • 特异性The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
  • 经测试应用适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human, Rhesus monkey
  • 免疫原

    A synthetic peptide corresponding to residues within human Flt3 ligand.

  • 阳性对照
    • IHC: Human tonsil tissue. WB: recombinant protein
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FL antibody
      • Flt 3 ligand antibody
      • Flt 3L antibody
      • Flt3 L antibody
      • FLT3 LG antibody
      • Flt3 ligand antibody
      • Flt3L antibody
      • FLT3L_HUMAN antibody
      • Flt3lg antibody
      • Fms related tyrosine kinase 3 ligand antibody
      • Fms-related tyrosine kinase 3 ligand antibody
      • SL cytokine antibody
      see all

    Anti-Flt3 ligand antibody [EP1140Y] 图像

    • ab52648 staining Flt3 ligand in Rhesus monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in 20% Normanl Goat serum in 1x TTBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Anti-Flt3 ligand antibody [EP1140Y] (ab52648) at 1/100000 dilution + Recombinant Flr3 ligand at 10 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 26 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.

    Anti-Flt3 ligand antibody [EP1140Y] (ab52648)参考文献

    This product has been referenced in:
    • Taylor RM  et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). Read more (PubMed: 22906984) »
    • Tobón GJ  et al. The Fms-like tyrosine kinase 3 ligand, a mediator of B cell survival, is also a marker of lymphoma in primary Sjögren's syndrome. Arthritis Rheum 62:3447-56 (2010). IF ; Human . Read more (PubMed: 20556820) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
    Sample Human Tissue sections (liver tumor)
    Specification liver tumor
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Dec 08 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rhesus monkey Tissue sections (Brain)
    Specification Brain
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C

    Abcam user community

    Verified customer

    提交于 Mar 28 2013