概述

  • 产品名称
    Anti-Flt3 ligand抗体
    参阅全部 Flt3 ligand 一抗
  • 描述
    兔多克隆抗体to Flt3 ligand
  • 经测试应用
    适用于: WB, Neutralising, Flow Cyt, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hflt3-Ligand (human flt3-Ligand)

  • 常规说明


    hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant Flt3 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.004 - 0.006 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml)
Flow Cyt Use at an assay dependent concentration. PubMed: 22897934
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. In conjunction with ab84261 rabbit polyclonal to Flt3 ligand (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt3 Ligand.

靶标

  • 功能
    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FL antibody
    • Flt 3 ligand antibody
    • Flt 3L antibody
    • Flt3 L antibody
    • FLT3 LG antibody
    • Flt3 ligand antibody
    • Flt3L antibody
    • FLT3L_HUMAN antibody
    • Flt3lg antibody
    • Fms related tyrosine kinase 3 ligand antibody
    • Fms-related tyrosine kinase 3 ligand antibody
    • SL cytokine antibody
    see all

图片

  • Flow cytometry analysis of Human Flt3 ligand-transfected mouse OP9 cell line, staining Flt3 ligand with ab9688. The primary antibody was conjugated to AlexaFluor®488 before incubation with paraformaldehyde-fixed, serum-blocked cells.

文献

This product has been referenced in:
  • Patel ES  et al. Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation. BMC Immunol 13:46 (2012). Flow Cyt ; Human . Read more (PubMed: 22897934) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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