RabMAb

Anti-Flotillin 1抗体[EP446Y] (ab78178)

概述

  • 产品名称Anti-Flotillin 1抗体[EP446Y]
    参阅全部 Flotillin 1 一抗
  • 描述
    兔单克隆抗体[EP446Y] to Flotillin 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Flotillin 1 aa 50-150.
    Database link: O75955

  • 阳性对照
    • K562 cell lysate. C2C12 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab78178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 48 kDa.

For unpurified, use 1/500 - 1/1000.

靶标

  • 功能May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • 序列相似性Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • 细胞定位Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLOT 1 antibody
    • FLOT1 antibody
    • FLOT1_HUMAN antibody
    • Flotillin-1 antibody
    • Flotillin1 antibody
    • Integral membrane component of caveolae antibody
    • Reggie 2 antibody
    see all

Anti-Flotillin 1 antibody [EP446Y] 图像

  • All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

    Lane 1 : mouse brain lysate at 10 µg
    Lane 2 : rat brain lysate at 20 µg
    Lane 3 : C6 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution (unpurified) + K562 cell lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

Anti-Flotillin 1 antibody [EP446Y] (ab78178)参考文献

ab78178 has not yet been referenced specifically in any publications.

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