antigen sequence: CQDSNICAVF AVQGGKVGRK HGIKRGRRPS IRSPAQRARG PWIHESKHPA FAKQQINLEM P, corresponding to N terminal amino acids 3-63 of Human FLJ42280.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FLJ42280 antibody (ab121627)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab121627 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLJ42280 antibody (ab121627)
ab121627 at 1/175 dilution staining FLJ42280 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in glandular cells.
has not yet been referenced specifically in any publications.
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