概述

  • 产品名称Anti-FLJ20530抗体
    参阅全部 FLJ20530 一抗
  • 描述
    兔多克隆抗体to FLJ20530
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide (Human) - the immunogen lies in the region between residues 200 and 250 of human FLJ20530/ KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656).

性能

应用

Our Abpromise guarantee covers the use of ab18050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 120 kDa.

靶标

  • 相关性FLJ20530 is a component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3' box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • C8orf52 antibody
    • Chromosome 8 open reading frame 52 antibody
    • FLJ20530 antibody
    • INT8 antibody
    • Integrator complex subunit 8 antibody
    • INTS 8 antibody
    • INTS8 antibody
    • KAONASHI protein 1 antibody
    • KAONASHI1 antibody
    • MGC131633 antibody
    see all

Anti-FLJ20530 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FLJ20530 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot using ab18050 at 0.2µg/ml on HeLa nuclear lysate.

    Lysate: 5µg/lane.
    Detection: Chemiluminescence with an exposure time of 20 minutes. Western blot using ab18050 at 0.2µg/ml on HeLa nuclear lysate.

    Lysate: 5µg/lane.
    Detection: Chemiluminescence with an exposure time of 20 minutes.

Anti-FLJ20530 antibody (ab18050)参考文献

ab18050 has not yet been referenced specifically in any publications.

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