Anti-FLJ14154抗体(ab103800)

概述

  • 产品名称Anti-FLJ14154抗体
    参阅全部 FLJ14154 一抗
  • 描述
    兔多克隆抗体to FLJ14154
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Chicken, Guinea pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 192-241 (DYIQHLGSAL ASLSPCSIPH RVYRQAHSLL CSFLPWSGIS SKSGIEYSRT ) of Human FLJ14154 (NP_001077070)

  • 阳性对照
    • U937 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

Anti-FLJ14154 antibody 图像

  • ab103800 staining FLJ14154 in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 2% BSA) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Anti-FLJ14154 antibody (ab103800) at 1 µg/ml + U937 Cell lysate at 10 µg

    Predicted band size : 27 kDa

Anti-FLJ14154 antibody (ab103800)参考文献

ab103800 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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提交于 Feb 27 2014

Western blot

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Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 Jun 04 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 Jun 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"