Full length protein, corresponding to amino acids 1-326 of Human FLJ10357, according to entry AAH00084.3
- FLJ10357-transfected 293T cell lysate
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 36 kDa.
Anti-FLJ10357 antibody 图像
Western blot - FLJ10357 antibody (ab68745)
All lanes :
Anti-FLJ10357 antibody (ab68745) at 1/500 dilutionLane 1 :
FLJ10357-transfected 293T cell lysateLane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.Secondary
Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilutionPredicted band size :
36 kDaObserved band size :
37 kDa (why is the actual band size different from the predicted?
Anti-FLJ10357 antibody (ab68745)参考文献
has not yet been referenced specifically in any publications.
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