概述

  • 产品名称Anti-FLIP抗体
    参阅全部 FLIP 一抗
  • 描述
    兔多克隆抗体to FLIP
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Human, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FLIP.

    (Peptide available as ab93738.)

  • 阳性对照
    • This antibody gave a positive signal in human skeletal muscle and placenta tissue lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab84283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human FLIP peptide (ab8457).
ICC/IF 1/200.

靶标

  • 功能Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • 组织特异性Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • 结构域The caspase domain lacks the active sites residues involved in catalysis.
  • 翻译后修饰Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c FLIP antibody
    • c FLIPL antibody
    • c FLIPR antibody
    • c FLIPS antibody
    • c-FLIP antibody
    • c-FLIPL antibody
    • c-FLIPR antibody
    • c-FLIPS antibody
    • CASH antibody
    • CASP8 and FADD like apoptosis regulator antibody
    • CASP8 and FADD-like apoptosis regulator subunit p12 antibody
    • CASP8AP1 antibody
    • Caspase homolog antibody
    • Caspase like apoptosis regulatory protein antibody
    • Caspase related inducer of apoptosis antibody
    • Caspase-eight-related protein antibody
    • Caspase-like apoptosis regulatory protein antibody
    • Casper antibody
    • Cellular FLICE-like inhibitory protein antibody
    • CFLA antibody
    • Cflar antibody
    • CFLAR_HUMAN antibody
    • CLARP antibody
    • FADD like anti apoptotic molecule antibody
    • FADD-like antiapoptotic molecule 1 antibody
    • FLAME antibody
    • FLAME-1 antibody
    • FLAME1 antibody
    • FLIP antibody
    • I FLICE antibody
    • I-FLICE antibody
    • Inhibitor of FLICE antibody
    • MACH-related inducer of toxicity antibody
    • MRIT antibody
    • OTTHUMP00000163715 antibody
    • OTTHUMP00000206475 antibody
    • OTTHUMP00000206476 antibody
    • OTTHUMP00000206478 antibody
    • OTTHUMP00000206479 antibody
    • OTTHUMP00000206480 antibody
    • OTTHUMP00000206482 antibody
    • OTTHUMP00000207360 antibody
    • Usurpin antibody
    • Usurpin beta antibody
    see all

Anti-FLIP antibody 图像

  • All lanes : Anti-FLIP antibody (ab84283) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab84283 (1/200) staining FLIP in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised using 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.

    See Abreview

Anti-FLIP antibody (ab84283)参考文献

ab84283 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"