Anti-FLIP抗体(ab8421)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-FLIP抗体
参阅全部 FLIP 一抗 -
描述
兔多克隆抗体to FLIP -
宿主
Rabbit -
特异性
Recognizes all FLIP splice variants including FLIP alpha, beta, gamma, and delta. -
经测试应用
适用于: WB, ICC/IF, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human FLIP aa 2-19.
Sequence:SAEVIHQVEEALDTDEKE
(Peptide available asab8457) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
FLIP Antibody is affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (10) |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
IHC-P |
Use a concentration of 5 µg/ml.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. -
组织特异性
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. -
序列相似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
结构域
The caspase domain lacks the active sites residues involved in catalysis. -
翻译后修饰
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. - Information by UniProt
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数据库链接
- Entrez Gene: 8837 Human
- Entrez Gene: 12633 Mouse
- Entrez Gene: 117279 Rat
- Omim: 603599 Human
- SwissProt: O15519 Human
- SwissProt: O35732 Mouse
- Unigene: 390736 Human
- Unigene: 336848 Mouse
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别名
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab8421 被引用在 21 文献中.
- Kamiya M et al. Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies. Nat Commun 13:166 (2022). PubMed: 35013338
- Ke C et al. Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib. Cancers (Basel) 12:N/A (2020). PubMed: 32375399
- Liu YP et al. circPVT1 Facilitates Invasion and Metastasis by Regulating miR-205-5p/c-FLIP Axis in Osteosarcoma. Cancer Manag Res 12:1229-1240 (2020). PubMed: 32110097
- Maiese A et al. Hypo-Expression of Flice-Inhibitory Protein and Activation of the Caspase-8 Apoptotic Pathways in the Death-Inducing Signaling Complex Due to Ischemia Induced by the Compression of the Asphyxiogenic Tool on the Skin in Hanging Cases. Diagnostics (Basel) 10:N/A (2020). PubMed: 33198065
- Ariana A et al. Tristetraprolin regulates necroptosis during tonic Toll-like receptor 4 (TLR4) signaling in murine macrophages. J Biol Chem 295:4661-4672 (2020). PubMed: 32094226