Anti-Flightless I抗体[EPR4202(2)] (ab109015)


  • 产品名称Anti-Flightless I抗体[EPR4202(2)]
    参阅全部 Flightless I 一抗
  • 描述
    兔单克隆抗体[EPR4202(2)] to Flightless I
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • Human heart, HeLa, A431, and A549 lysates; Human muscle tissue; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab109015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
    • 组织特异性Strongest expression in skeletal muscle with high expression also in the heart and lung.
    • 疾病相关Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
    • 序列相似性Contains 5 gelsolin-like repeats.
      Contains 15 LRR (leucine-rich) repeats.
    • 细胞定位Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Fli 1 antibody
      • FLI antibody
      • Fli1 antibody
      • Flightless 1 antibody
      • Flightless I (Drosophila) homolog antibody
      • Flightless I homolog antibody
      • Flightless I homolog (Drosophila) antibody
      • Flightless1 antibody
      • FlightlessI antibody
      • fliI antibody
      • FLII_HUMAN antibody
      • Fliih antibody
      • FLIL antibody
      • MGC39265 antibody
      • Protein flightless 1 homolog antibody
      • Protein flightless-1 homolog antibody
      see all

    Anti-Flightless I antibody [EPR4202(2)] 图像

    • All lanes : Anti-Flightless I antibody [EPR4202(2)] (ab109015) at 1/1000 dilution

      Lane 1 : Humam heart lysate
      Lane 2 : HeLa lysate
      Lane 3 : A431 lysate
      Lane 4 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 145 kDa
    • Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.
    • Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution.

    Anti-Flightless I antibody [EPR4202(2)] (ab109015)参考文献

    ab109015 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109015.
    Please use the links above to contact us or submit feedback about this product.