Anti-FLI1抗体- ChIP Grade (ab15289)

概述

  • 产品名称
    Anti-FLI1抗体- ChIP Grade
    参阅全部 FLI1 一抗
  • 描述
    兔多克隆抗体to FLI1 - ChIP Grade
  • 经测试应用
    适用于: ChIP, WB, IP, CHIPseq, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • 阳性对照
    • Angiosarcoma

性能

应用

Our Abpromise guarantee covers the use of ab15289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 19289502
WB Use a concentration of 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP Use a concentration of 2 µg/ml.
CHIPseq Use at an assay dependent concentration. PubMed: 24760698
IHC-P 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 疾病相关
    Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • 序列相似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • Fli-1 proto-oncogene, ETS transcription factor antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 (FLI1) transcription factor antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • Sarcoma breakpoint region 2 (EWSR2) antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    • Viral integration region FLI1, mouse, homolog of antibody
    see all

图片

  • Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 0.4% and 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.

    See Abreview

  • All lanes : Anti-FLI1 antibody - ChIP Grade (ab15289) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab15289, at 1/50 dilution, staining FLI1 in angiosarcoma by Immunohistochemistry (FFPE-sections).

文献

This product has been referenced in:
  • Gardiner JD  et al. C/EBPß-1 promotes transformation and chemoresistance in Ewing sarcoma cells. Oncotarget 8:26013-26026 (2017). Read more (PubMed: 28148901) »
  • Schachterle W  et al. Sox17 drives functional engraftment of endothelium converted from non-vascular cells. Nat Commun 8:13963 (2017). Read more (PubMed: 28091527) »

See all 20 Publications for this product

客户评价及客户问答

The concentration of Anti-FLI1 antibody - ChIP Grade (ab15289) lot GR320740-1 is 0.105 mg/ml.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Aorta)
Permeabilization
Yes - 0.5%TritonX-100
Specification
Aorta
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 14 2016

Thank you for contacting us.
Unfortunately the exact immunogen sequence is confidential. I can tell you it is located within aa 385-452 of Q01543 (Uniprot).

I hope this information is helpful to you. Please do not hesitate to contact us...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification
haematopoietic progenitor cell line HPC-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: both 0.4% and 1% formaldehyde
Detection step
Real-time PCR
Positive control
i) a competitor antibody known to work
ii) a regulatory region known to bind this transcription factor
Negative control
a known region this transcription factor does not bind
Username

Abcam user community

Verified customer

提交于 Oct 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (macrophage)
Total protein in input
100 µg
Specification
macrophage
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Apr 24 2006

Thank you for your enquiry and your interest in our products. The antigens for these two antibodies are the same, however ab15290 is prediluted so so you use it without any further dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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