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Our Abpromise guarantee covers the use of ab15289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 19289502|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IP||Use a concentration of 2 µg/ml.|
|CHIPseq||Use at an assay dependent concentration. PubMed: 24760698|
|IHC-P||1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 0.4% and 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.
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