概述

  • 产品名称Anti-FLI1抗体
    参阅全部 FLI1 一抗
  • 描述
    兔多克隆抗体to FLI1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human FLI1.

  • 阳性对照
    • This antibody gave a positive signal in MEF1 and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab90583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).

靶标

  • 功能Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 疾病相关Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    see all

Anti-FLI1 antibody 图像

  • All lanes : Anti-FLI1 antibody (ab90583) at 1 µg/ml

    Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa,31 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-FLI1 antibody (ab90583)参考文献

ab90583 has not yet been referenced specifically in any publications.

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