概述

  • 产品名称Anti-FLI1抗体
    参阅全部 FLI1 一抗
  • 描述
    兔多克隆抗体to FLI1
  • 特异性FLI1 antibody detects endogenous levels of total FLI1 protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from human FLI1

  • 阳性对照
    • human breast carcinoma tissue; NIH/3T3 cell extract

性能

应用

Our Abpromise guarantee covers the use of ab53077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 疾病相关Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    see all

Anti-FLI1 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53077 at 1/50 dilution, with and without immunizing peptide.
  • All lanes : Anti-FLI1 antibody (ab53077) at 1/50 dilution

    Lane 1 : NIH/3T3 cell extract with no immunizing peptide
    Lane 2 : NIH/3T3 cell extract with immunizing peptide


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

Anti-FLI1 antibody (ab53077)参考文献

ab53077 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification haematopoietic progenitor cell line HPC-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: both 0.4% and 1% formaldehyde
Detection step Real-time PCR
Positive control i) a competitors antibody known to work
ii) regulatory region known to bind this transcription factor
Negative control a known region that this transcription factor does not bind
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提交于 Oct 06 2009

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