概述

  • 产品名称Anti-FLI1抗体
    参阅全部 FLI1 一抗
  • 描述
    兔多克隆抗体to FLI1
  • 特异性This antibody recognizes FLI1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    A region within synthetic peptide: SGSPDYGQPH KINPLPPQQE WINQPVRVNV KREYDHMNGS RESPVDCSVS, corresponding to N terminal amino acids 37-86 of Human FLI1

  • 阳性对照
    • Jurkat cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab49045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 疾病相关Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    see all

Anti-FLI1 antibody 图像

  • Lane 1 : MARKER
    Lane 2 : Anti-FLI1 antibody (ab49045) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-FLI1 antibody (ab49045) at 1/1000 dilution

    Lane 1 : Cytoplasmic lysate prepared from porcine endothelial cells
    Lane 2 : Nuclear lysate prepared from porcine endothelial cells

    Secondary
    Biotin conjugated anti-rabbit at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa


    Exposure time : 1 minute

Anti-FLI1 antibody (ab49045)参考文献

ab49045 has not yet been referenced specifically in any publications.

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