Anti-FLG2抗体(ab122011)

概述

  • 产品名称
    Anti-FLG2抗体
  • 描述
    兔多克隆抗体to FLG2
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    amino acids 280-381 of Human FLG2 (Q5D862).

  • 阳性对照
    • Human prostate tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

  • 序列相似性
    Belongs to the S100-fused protein family.
    In the N-terminal section; belongs to the S-100 family.
    Contains 2 EF-hand domains.
    Contains 10 filaggrin repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FILA2_HUMAN antibody
    • Filaggrin-2 antibody
    • FLG-2 antibody
    • FLG2 antibody
    • Ifapsoriasin antibody
    • Intermediate filament-associated and psoriasis-susceptibility protein antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

文献

ab122011 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Loading amount
14 µg
Gel Running Conditions
Reduced Denaturing (gel 10%)
Sample
Human Serum (plasma)
Specification
plasma
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Miss. Yi-Tsen Chiang

Verified customer

提交于 Jan 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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