Anti-FLG2抗体(ab122011)

Abreviews (1) Submit a Question
Customer reviews and FAQs

概述

  • 产品名称Anti-FLG2抗体
  • 描述
    兔多克隆抗体to FLG2
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    amino acids 280-381 of Human FLG2 (Q5D862).

  • 阳性对照
    • Human prostate tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

  • 序列相似性Belongs to the S100-fused protein family.
    In the N-terminal section; belongs to the S-100 family.
    Contains 2 EF-hand domains.
    Contains 10 filaggrin repeats.
  • Information by UniProt
  • 数据库链接
    • 别名
      • FILA2_HUMAN antibody
      • Filaggrin-2 antibody
      • FLG-2 antibody
      • FLG2 antibody
      • Ifapsoriasin antibody
      • Intermediate filament-associated and psoriasis-susceptibility protein antibody
      see all

    Anti-FLG2 antibody 图像

    • Immunocytochemistry/ Immunofluorescence - Anti-FLG2 antibody (ab122011)
      Immunocytochemistry/ Immunofluorescence - Anti-FLG2 antibody (ab122011)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLG2 antibody (ab122011)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLG2 antibody (ab122011)
      ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

    Anti-FLG2 antibody (ab122011)参考文献

    ab122011 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab122011？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab122011 的 abreview 提交 ab122011 的问题

    1 Abreview or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-FLG2 antibody

     Below Average
    Abreviews
    Application Western blot
    Loading amount 14 µg
    Gel Running Conditions Reduced Denaturing (gel 10%)
    Sample Human Serum (plasma)
    Specification plasma
    Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    abreview image
    Username

    Miss. Yi-Tsen Chiang

    Verified customer

    提交于 Jan 02 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"