概述

  • 产品名称Anti-FLCN抗体
    参阅全部 FLCN 一抗
  • 描述
    兔多克隆抗体to FLCN
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the center region of Human FLCN (NP_659434.2).

  • 阳性对照
    • CEM cell line lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab93196 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 64 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能May play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). May be a tumor suppressor. May be involved in colorectal tumorigenesis. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
  • 组织特异性Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.
  • 疾病相关Defects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD) [MIM:135150]. BHD is a rare autosomal dominant genodermatosis characterized by hair follicle hamartomas (fibrofolliculomas), kidney tumors, and spontaneous pneumothorax. Fibrofolliculomas are part of the triad of BHD skin lesions that also includes trichodiscomas and acrochordons. Onset of this dermatologic condition is invariably in adulthood. BHD is associated with a variety of histologic types of renal tumors, including chromophobe renal cell carcinoma (RCC), benign renal oncocytoma, clear-cell RCC and papillary type I RCC. Multiple lipomas, angiolipomas, and parathyroid adenomas are also seen in patients affected with this disease. The majority of mutations are predicted to prematurely terminate the protein.
    Defects in FLCN are in some cases a cause of primary spontaneous pneumothorax (PSP) [MIM:173600]. PSP is a condition in which air is present in the pleural space in the absence of a precipitating event, such as trauma or lung disease. This results in secondary collapse of the lung, either partially or completely, and some degree of hypoxia. PSP is relatively common, with an incidence between 7.4-18/100'000 for men and 1.2-6/100'000 for women and a dose-dependent, increased risk among smokers. Most cases are sporadic, typically occurring in tall, thin men aged 10-30 years and generally while at rest. Familial PSP is rarer and usually is inherited as an autosomal dominant condition with reduced penetrance, although X-linked recessive and autosomal recessive inheritance have also been suggested.
    Note=Defects in FLCN may be involved in renal cell carcinoma.
  • 序列相似性Belongs to the folliculin family.
  • 发展阶段Expressed in fetal lung, kidney, liver, and brain.
  • 翻译后修饰Phosphorylated. Several different phosphorylated forms exist.
  • 细胞定位Cytoplasm. Nucleus. Mainly localized in the nucleus. Co-localizes with FNIP1 and FNIP2 in the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BHD antibody
    • BHD skin lesion fibrofolliculoma protein antibody
    • Birt Hogg Dube syndrome protein antibody
    • Birt-Hogg-Dube syndrome protein antibody
    • DKFZp547A118 antibody
    • FLCL antibody
    • Flcn antibody
    • FLCN_HUMAN antibody
    • FLJ45004 antibody
    • FLJ99377 antibody
    • Folliculin antibody
    • MGC17998 antibody
    • MGC23445 antibody
    see all

Anti-FLCN antibody 图像

  • Anti-FLCN antibody (ab93196) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 64 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma, staining FLCN with ab93196 at 1/50 dilution. Staining was detected using DAB.

Anti-FLCN antibody (ab93196)参考文献

ab93196 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (BHD kidney tumor cell lines: UOK257 (FLCN-) & UOK2)
Gel Running Conditions Reduced Denaturing (Bio-Rad 8-16% gradient Tris-Glycine gel)
Loading amount 25 µg
Specification BHD kidney tumor cell lines: UOK257 (FLCN-) & UOK2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 May 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"