Anti-Flavivirus E-glycoprotein抗体[HF8-1/C1-6/E12] (ab214335)
Key features and details
- Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein
- Suitable for: WB, ICC/IF, ELISA, Sandwich ELISA
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
概述
-
产品名称
Anti-Flavivirus E-glycoprotein抗体[HF8-1/C1-6/E12]
参阅全部 Flavivirus E-glycoprotein 一抗 -
描述
小鼠单克隆抗体[HF8-1/C1-6/E12] to Flavivirus E-glycoprotein -
宿主
Mouse -
特异性
ab214335 reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV. -
经测试应用
适用于: WB, ICC/IF, ELISA, Sandwich ELISAmore details -
种属反应性
与反应: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus -
免疫原
Recombinant full length protein corresponding to Dengue virus 1 Flavivirus E-glycoprotein aa 250-800.
Database link: P27909 -
阳性对照
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640 -
Concentration information loading...
-
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
HF8-1/C1-6/E12 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab214335于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
|
ICC/IF |
Use at an assay dependent concentration.
Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
|
ELISA |
Use at an assay dependent concentration.
This monoclonal antibody has been primarily used for the capture/detection of DENV1, 2, 3, 4, ZIKV (data not shown), JEV and WNV antigens in an antigen capture ELISA. |
|
Sandwich ELISA |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use at an assay dependent concentration. Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
ICC/IF
Use at an assay dependent concentration. Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
ELISA
Use at an assay dependent concentration. This monoclonal antibody has been primarily used for the capture/detection of DENV1, 2, 3, 4, ZIKV (data not shown), JEV and WNV antigens in an antigen capture ELISA. |
Sandwich ELISA
Use at an assay dependent concentration. |
靶标
-
相关性
The Flaviviridae are a family of viruses that are primarily spread through arthropod vectors (mainly ticks and mosquitoes). Flaviviridae have monopartite, linear, single-stranded RNA genomes of positive polarity, 9.6- to 12.3-kilobase in length with enveloped and spherical virus particles, about 40-60 nm in diameter. -
数据库链接
- SwissProt: P27909 Dengue virus 1
- SwissProt: P29990 Dengue virus 2
- SwissProt: P29991 Dengue virus 2
- SwissProt: Q6YMS4 Dengue virus 3
- SwissProt: Q2YHF0 Dengue virus 4
- SwissProt: P14403 Japanese encephalitis virus
- SwissProt: P06935 West Nile virus
- SwissProt: Q32ZE1 Zika virus
-
别名
- Flavivirus envelope glycoprotein antibody
图片
-
Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214335.
-
ELISA analysis using Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
-
Sandwich ELISA detection Flavivirus E-glycoprotein in Zika infected and non infected C6/36 cells using ab214335 and other anti Flavivirus E-glycoprotein antibodies as capture antibody.
-
All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : C6/36 cell lysate control
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab214335 尚未被引用在任何文献中。