概述

  • 产品名称
  • 描述
    兔多克隆抗体to FLASH
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    SERFQQLMKLFEKSKC

    , corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.)

  • 阳性对照
    • HeLa cell lysate An approximate 220 kDa band can be detected.
  • 常规说明


    A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
  • 克隆
    多克隆
  • 同种型
    IgG
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Nucleus. Localizes to discrete nuclear foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA387232 antibody
    • C8AP2_HUMAN antibody
    • CASP 8 associated protein 2 antibody
    • CASP8 associated protein 2 antibody
    • CASP8-associated protein 2 antibody
    • CASP8AP2 antibody
    • Caspase 8 associated protein 2 antibody
    • CED 4 antibody
    • CED4 antibody
    • D4Ertd659e antibody
    • FLASH homolog antibody
    • FLASH homolog RIP 25 antibody
    • FLASH homolog RIP25 antibody
    • FLICE associated huge antibody
    • FLICE associated huge protein antibody
    • FLICE-associated huge protein antibody
    • FLJ11208 antibody
    • KIAA1315 antibody
    • mKIAA1315 antibody
    • OTTMUSP00000005125 antibody
    • RIP 25 antibody
    • RIP25 antibody
    • RP23-69G24.1 antibody
    see all

图片

  • Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate

    Observed band size : 220 kDa (why is the actual band size different from the predicted?)
  • ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris-acetate)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2008

Our FLASH antibodies have not yet been tested for IHC and if you wish to test ab8420 for use in immmunocytochemistry, I would be happy to offer you a 30% discount in exchange for IHC information. Please quote reference SMDD1609-30% when placing your or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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