概述

  • 产品名称Anti-FLASH抗体
    参阅全部 FLASH 一抗
  • 描述
    兔多克隆抗体to FLASH
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    SERFQQLMKLFEKSKC

    , corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.)

  • 阳性对照
    • HeLa cell lysate An approximate 220 kDa band can be detected.
  • 常规说明


    A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液PBS with 0.02% sodium azide
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab8420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus. Localizes to discrete nuclear foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA387232 antibody
    • C8AP2_HUMAN antibody
    • CASP 8 associated protein 2 antibody
    • CASP8 associated protein 2 antibody
    • CASP8-associated protein 2 antibody
    • CASP8AP2 antibody
    • Caspase 8 associated protein 2 antibody
    • CED 4 antibody
    • CED4 antibody
    • D4Ertd659e antibody
    • FLASH homolog antibody
    • FLASH homolog RIP 25 antibody
    • FLASH homolog RIP25 antibody
    • FLICE associated huge antibody
    • FLICE associated huge protein antibody
    • FLICE-associated huge protein antibody
    • FLJ11208 antibody
    • KIAA1315 antibody
    • mKIAA1315 antibody
    • OTTMUSP00000005125 antibody
    • RIP 25 antibody
    • RIP25 antibody
    • RP23-69G24.1 antibody
    see all

Anti-FLASH antibody 图像

  • Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate

    Observed band size : 220 kDa (why is the actual band size different from the predicted?)
  • ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF

Anti-FLASH antibody (ab8420)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount 10 µg
Specification LNCaP (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (7% tris-acetate)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Jul 02 2008

Our FLASH antibodies have not yet been tested for IHC and if you wish to test ab8420 for use in immmunocytochemistry, I would be happy to offer you a 30% discount in exchange for IHC information. Please quote reference SMDD1609-30% when placing your or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"