Anti-FLAP抗体(ab85227)
Key features and details
- Rabbit polyclonal to FLAP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FLAP抗体
参阅全部 FLAP 一抗 -
描述
兔多克隆抗体to FLAP -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab85227 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab124714.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Cow, Macaque monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in Human small intestine tissue lysate as well as the following whole cell lysates: U937; THP1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. -
疾病相关
Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. -
序列相似性
Belongs to the MAPEG family. -
结构域
The C-terminal part after residue 140 is mostly unstructured. -
细胞定位
Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 100034178 Horse
- Entrez Gene: 241 Human
- Entrez Gene: 11690 Mouse
- Entrez Gene: 100009413 Rabbit
- Omim: 603700 Human
- SwissProt: P30353 Horse
- SwissProt: P20292 Human
- SwissProt: P30355 Mouse
see all -
别名
- 5-lipoxygenase activating protein antibody
- AL5AP_HUMAN antibody
- ALOX 5AP antibody
see all
图片
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ab85227 staining FLAP in HDLM-2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85227 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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All lanes : Anti-FLAP antibody (ab85227) at 1 µg/ml
Lane 1 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 175 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab85227 被引用在 16 文献中.
- Miek L et al. Staphylococcus aureus controls eicosanoid and specialized pro-resolving mediator production via lipoteichoic acid. Immunology 166:47-67 (2022). PubMed: 35143048
- Zhang K et al. Modulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization. J Inflamm Res 15:3285-3304 (2022). PubMed: 35676971
- Troisi F et al. Sex Hormone-Dependent Lipid Mediator Formation in Male and Female Mice During Peritonitis. Front Pharmacol 12:818544 (2021). PubMed: 35046831
- Rao Z et al. Differential role of vacuolar (H+)-ATPase in the expression and activity of cyclooxygenase-2 in human monocytes. Biochem Pharmacol 175:113858 (2020). PubMed: 32061774
- Gilbert NC et al. Structural and mechanistic insights into 5-lipoxygenase inhibition by natural products. Nat Chem Biol 16:783-790 (2020). PubMed: 32393899