概述

  • 产品名称
    Anti-Flagellin抗体
    参阅全部 Flagellin 一抗
  • 描述
    兔多克隆抗体to Flagellin
  • 宿主
    Rabbit
  • 特异性
    This antibody is specific for bacterial Flagellin (FliC).
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Escherichia coli, Salmonella anatum, Salmonella selandia
  • 免疫原

    Purified recombinant Flagellin made in BL21 bacteria.

  • 阳性对照
    • Lysates transfected with purified flagellin, E. Coli total lysate or 293T transfected with recombinant protein.

性能

应用

Our Abpromise guarantee covers the use of ab93713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 60 kDa.
ICC/IF 1/100.

靶标

  • 相关性
    Flagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs).
  • 细胞定位
    Secreted. Bacterial flagellum.
  • 数据库链接
    • 别名
      • 41 kDa antigen antibody
      • b1923 antibody
      • flaF antibody
      • Flagellar filament 41 kDa core protein antibody
      • Flagellin antibody
      • fliC antibody
      • hag antibody
      • JW1908 antibody
      • P41 antibody
      see all

    图片

    • All lanes : Anti-Flagellin antibody (ab93713) at 1/10000 dilution

      All lanes : 293T transfected with recombinant protein

      Observed band size: 60 kDa (why is the actual band size different from the predicted?)

    • Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.

      Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.
    • Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.

      Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.

    文献

    This product has been referenced in:
    • Stepanova LA  et al. Protection against multiple influenza A virus strains induced by candidate recombinant vaccine based on heterologous M2e peptides linked to flagellin. PLoS One 10:e0119520 (2015). WB, ELISA . Read more (PubMed: 25799221) »
    • Zhang W  et al. Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP. Mol Cancer 13:128 (2014). WB . Read more (PubMed: 24886421) »

    See all 3 Publications for this product

    客户评价及客户问答

    Thank you for contacting us.

    Unfortunately, I do not know which bacteria you are investigating. Ifyou are looking at peritrichous bacteria such as E.coli or salmonella, the flagella are all over the bacteria, so the antibody is going to light...

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    Application
    Western blot
    Sample
    Salmonella typhi Cell lysate - other (Flagellin preparation of whole cell cultures)
    Gel Running Conditions
    Reduced Denaturing (12% SDS-Page gel)
    Loading amount
    100 µg
    Specification
    Flagellin preparation of whole cell cultures
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Amanda Gonzales

    Verified customer

    提交于 Feb 28 2018

    Thank you for getting in touch.


    Please find below the protocols for infecting macrophages with salmonella and the protocol for staining the infected macrophages.


    I wish you successful experiments.


    Salmonella in...

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    Thank you for contacting us.

    The flagellin antibody was generated against recombinant purified
    full-length FliC from E Coli DH5a. There are similarities at the N and C
    termini with P aeruginosa FliC but it is probably not enough fo...

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    Thank you for contacting us and thank you for your patience.

    The protocol seems fairly standard. However, she was in contact with the originator of this antibody, and here aretheir thoughts:

    1) The rods are certainly full of newly e...

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    Thank you for your call earlier and for your patience while I have looked into your enquiry.

    Based on the sequences available on UniProt, I don't expect any of our flagellin antibodies to react with pseudomonas samples. There were multiple pu...

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    Thank you for contacting Abcam. The concentration of protein in ab93713 is between 30 and 40 mg/ml. We do not know how much of that total is made up with antibody because the sample is serum, not purified antibody. Please let me know if there is anythi...

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    Thank you for contacting us. The total protein concentration of ab93713, lot GR35089-1, is around 30-40 mg/ml. However, since this is whole serum containing a wide variety of proteins, the concentration of the specific anti-Flagellin antibody canno...

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    J'ai été informé par laboratoire que l'anticorps ab93713 peut en effet détecter la flagelline de Salmonella typhimurium. Malheureusement ils n'ont jamais testé la réactivité de l'anticorps avec bacilis subtilis. Comme expliqué par téléphone, nous n’off...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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