The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: Use at a concentration of 10 µg/ml. Note: ab89096 requires purification prior to use in ICC/IF.
WB: 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性FKSG14, also known as CENPK (centromere protein K) is a component of the CENPA-CAD (nucleosome distal) complex. It may be involved in incorporation of CENPA into centromeres and is required for proper kinetochore function, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. FKSG14 constitutively localized to centromeres throughout the cell cycle. There are 3 isoforms produced by alternative splicing.
细胞定位Nuclear. Centromere. Kinetochore. Localizes exclusively in the centromeres.