Recombinant fragment with tag: TYFKSSHLKA HLRTHTGEKP FNCSWDGCDK KFARSDELSR HRRTHTGEKK FVCPVCDRRF MRSDHLTKHA RRHMTTKKIP GWQAEVGKLN RIASAESPGS PLVSMPASA, corresponding to amino acids 404-513 of Human FKLF/ KLF11
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Use at an assay dependent concentration.
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis.
组织特异性Ubiquitous. Higher expression in erythroid cells.
疾病相关Defects in KLF11 are the cause of maturity-onset diabetes of the young type 7 (MODY7) [MIM:610508]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Overlay histogram showing K562 cells stained with ab61207 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61207, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - FKLF / KLF11 antibody (ab61207)
Anti-FKLF / KLF11 antibody (ab61207) at 1 µg/ml + HeLa nuclear extract at 50 µg
Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 56 kDa Observed band size : 56 kDa
Anti-FKLF / KLF11 antibody (ab61207)参考文献
has not yet been referenced specifically in any publications.