Anti-FKLF / KLF11抗体(ab61207)

概述

  • 产品名称Anti-FKLF / KLF11抗体
    参阅全部 FKLF / KLF11 一抗
  • 描述
    小鼠单克隆抗体to FKLF / KLF11
  • 经测试应用适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment with tag: TYFKSSHLKA HLRTHTGEKP FNCSWDGCDK KFARSDELSR HRRTHTGEKK FVCPVCDRRF MRSDHLTKHA RRHMTTKKIP GWQAEVGKLN RIASAESPGS PLVSMPASA, corresponding to amino acids 404-513 of Human FKLF/ KLF11

  • 阳性对照
    • HeLa cell nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab61207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis.
  • 组织特异性Ubiquitous. Higher expression in erythroid cells.
  • 疾病相关Defects in KLF11 are the cause of maturity-onset diabetes of the young type 7 (MODY7) [MIM:610508]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9830142A17 antibody
    • D12Ertd427e antibody
    • FKLF antibody
    • FKLF1 antibody
    • Klf11 antibody
    • KLF11_HUMAN antibody
    • Krueppel like factor 11 antibody
    • Krueppel-like factor 11 antibody
    • MODY7 antibody
    • Tcfcp2l2 antibody
    • TGFB Early Growth Response 2 antibody
    • TGFB-inducible early growth response protein 2 antibody
    • TGFB-inducible early growth response protein 2b antibody
    • TGFB-inducible early growth response protein 3 antibody
    • TIEG 2 antibody
    • TIEG-2 antibody
    • TIEG-3 antibody
    • Tieg2b antibody
    • Transforming Growth Factor Beta Inducible Early Growth Response 2 antibody
    • Transforming growth factor-beta-inducible early growth response protein 2 antibody
    • Transforming growth factor-beta-inducible early growth response protein 3 antibody
    see all

Anti-FKLF / KLF11 antibody 图像

  • Overlay histogram showing K562 cells stained with ab61207 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61207, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-FKLF / KLF11 antibody (ab61207) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

Anti-FKLF / KLF11 antibody (ab61207)参考文献

ab61207 has not yet been referenced specifically in any publications.

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