使用敲除细胞株进行验证

Anti-FKBP51抗体[Hi51B] (ab79844)

概述

  • 产品名称
    Anti-FKBP51抗体[Hi51B]
    参阅全部 FKBP51 一抗
  • 描述
    小鼠单克隆抗体[Hi51B] to FKBP51
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Hamster, Dog, Human
  • 免疫原

    Full length Human FKBP51, binding epitope in TPR domain

  • 阳性对照
    • WB: HeLa cell lysate, rat kidney and rat and mouse testes tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab79844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/2000. Predicted molecular weight: 51 kDa.

靶标

  • 功能
    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • 组织特异性
    Widely expressed, enriched in testis compared to other tissues.
  • 序列相似性
    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 51 kDa FK506 binding protein 5 antibody
    • 51 kDa FK506 binding protein antibody
    • 51 kDa FK506-binding protein antibody
    • 51 kDa FKBP antibody
    • 54 kDa progesterone receptor associated immunophilin antibody
    • 54 kDa progesterone receptor-associated immunophilin antibody
    • AIG 6 antibody
    • AIG6 antibody
    • Androgen regulated protein 6 antibody
    • Androgen-regulated protein 6 antibody
    • FF1 antigen antibody
    • FK506 binding protein 5 antibody
    • FK506-binding protein 5 antibody
    • FKBP 5 antibody
    • FKBP 51 antibody
    • FKBP 54 antibody
    • FKBP-5 antibody
    • FKBP-51 antibody
    • FKBP5 antibody
    • FKBP5_HUMAN antibody
    • FKBP54 antibody
    • HSP90 binding immunophilin antibody
    • HSP90-binding immunophilin antibody
    • MGC111006 antibody
    • OTTHUMP00000016268 antibody
    • P54 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP5 antibody
    • Ptg 10 antibody
    • Ptg10 antibody
    • Rotamase antibody
    • T cell FK506 binding protein antibody
    see all

图片

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab79844 observed at 51 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab79844 was shown to recognize FKBP51 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. ab79844 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ab79844 at 1/100 dilution staining FKBP51 in normal MK cells (IF localization shown in red) by Immunocytochemistry/ immunofluorescence.

文献

ab79844 has not yet been referenced specifically in any publications.

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