概述

  • 产品名称Anti-FKBP51抗体
    参阅全部 FKBP51 一抗
  • 描述
    兔多克隆抗体to FKBP51
  • 经测试应用适用于: IP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human FKBP51.

    (Peptide available as ab46177)

  • 阳性对照
    • ab46002 gave a positive result in the following Human Whole Cell Lysates: Jurkat HL60

性能

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应用

Our Abpromise guarantee covers the use of ab46002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
ICC/IF 1/100.

靶标

  • 功能Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • 组织特异性Widely expressed, enriched in testis compared to other tissues.
  • 序列相似性Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 51 kDa FK506 binding protein 5 antibody
    • 51 kDa FK506 binding protein antibody
    • 51 kDa FK506-binding protein antibody
    • 51 kDa FKBP antibody
    • 54 kDa progesterone receptor associated immunophilin antibody
    • 54 kDa progesterone receptor-associated immunophilin antibody
    • AIG 6 antibody
    • AIG6 antibody
    • Androgen regulated protein 6 antibody
    • Androgen-regulated protein 6 antibody
    • FF1 antigen antibody
    • FK506 binding protein 5 antibody
    • FK506-binding protein 5 antibody
    • FKBP 5 antibody
    • FKBP 51 antibody
    • FKBP 54 antibody
    • FKBP-5 antibody
    • FKBP-51 antibody
    • FKBP5 antibody
    • FKBP5_HUMAN antibody
    • FKBP54 antibody
    • HSP90 binding immunophilin antibody
    • HSP90-binding immunophilin antibody
    • MGC111006 antibody
    • OTTHUMP00000016268 antibody
    • P54 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP5 antibody
    • Ptg 10 antibody
    • Ptg10 antibody
    • Rotamase antibody
    • T cell FK506 binding protein antibody
    see all

Anti-FKBP51 antibody 图像

  • All lanes : Anti-FKBP51 antibody (ab46002) at 1/250 dilution

    Lane 1 : Jurkat whole cell lysate (ab7899)
    Lane 2 : HL60 whole cell lysate (ab7914)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 51 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • FKBP51 was immunoprecipitated using 0.5mg HL60 whole cell extract, 5µg of Rabbit polyclonal to FKBP51 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HL60 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab46002.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa: FKBP51.
  • IHC image of FKBP51 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46002, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab46002 staining human U2OS, mouse MEF, and rat RIE1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.

    See Abreview

Anti-FKBP51 antibody (ab46002)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human (U2OS) mouse (MEF) rat (RIE1))
Specification human (U2OS) mouse (MEF) rat (RIE1)
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100 [PBS]
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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提交于 Apr 10 2008

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