Anti-FKBP135抗体(ab14433)

概述

  • 产品名称Anti-FKBP135抗体
    参阅全部 FKBP135 一抗
  • 描述
    兔多克隆抗体to FKBP135
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-Fr, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa960 and aa1020 of human FKBP135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).

  • 阳性对照
    • HeLa cells.
  • 常规说明Centrifuge tube to remove product from lid.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Tris-citrate/phosphate. pH 7-8.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibody was purified using an epitope specific to FKBP135 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
WB
IP
IHC-P
  • 应用说明IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use at a concentration of 1 to 4 mcg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 148 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    Prepare working dilution immediately before use.
  • 靶标

    • 功能May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.
    • 序列相似性Belongs to the FKBP-type PPIase family.
      Contains 1 PPIase FKBP-type domain.
    • 结构域The PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
      The central coiled-coil region is responsible for association with early endosomes.
    • 细胞定位Cytoplasm. Cell projection > axon. Early endosome. Present in axons and neuronal growth cones.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 133 kDa FK506-binding protein antibody
      • 133 kDa FKBP antibody
      • C430014M02Rik antibody
      • FK506 binding protein 135 antibody
      • FK506-binding protein 15 antibody
      • FKB15_HUMAN antibody
      • FKBO133 antibody
      • FKBP-133 antibody
      • FKBP-15 antibody
      • FKBP133 antibody
      • Fkbp15 antibody
      • KIAA0674 antibody
      • mKIAA0674 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • RIKEN cDNA C430014M02 gene antibody
      • RP23 64F17.7 antibody
      • WAFL antibody
      • WASP and FKBP-like antibody
      see all

    Anti-FKBP135 antibody 图像

    • Detection of Human FKBP-135 by Western Blot and Immunoprecipitation. Samples: Nuclear extract (5 and 20 mcg for WB; 1 mg for IP) from HeLa cells. Antibodies: ab14432 used at 0.2 mcg/ml for WB in lane 1,2,3,4,5 and at 2 µg/mg extract for IP in lane 3 (B), ab14433 used at 2 µg/mg extract for IP in lane 4 (B) and ab14434 used at 2 mcg/mg extract for IP in lane 5 (B). Detection: chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).

    Anti-FKBP135 antibody (ab14433)参考文献

    ab14433 has not yet been referenced specifically in any publications.

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    I am sorry if it was misleading but the image supplied with Anti-FKBP135 antibodies ab14432, ab14433 and ab14434 are the same. They illustrate the IP performed with ab14432,...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 1X
    Permeabilization No
    Blocking step Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    提交于 Jan 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Penile Tissue)
    Specification Penile Tissue
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.4% Triton/PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15µg/mL · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    提交于 Dec 10 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Penile Tissue Sections)
    Specification Penile Tissue Sections
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 1X
    Permeabilization Yes - TBST 15 minutes
    Blocking step Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    提交于 Dec 10 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Penile Tissue)
    Loading amount 60 µg
    Specification Penile Tissue
    Gel Running Conditions Reduced Denaturing
    Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    提交于 Sep 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"