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Our Abpromise guarantee covers the use of ab14432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 135 kDa.|
|IP||Use a concentration of 1 - 4 mg/ml.|
ab14432 staining FKBP135 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. An Alexa Fluor®597-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Cells labelled for FKBP15 displayed punctate staining, which was absent in cells treated with siRNA against FKBP15.
Antibodies: ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection: Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"