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Our Abpromise guarantee covers the use of ab80264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23922772|
|WB||1/500 - 1/5000. Predicted molecular weight: 49 kDa.|
ab80264 staining FJX1 by ICC/IF (Immunocytochemistry/immunofluorescence) in HEK293T cells stably expressing MYC-tagged FJX1 dual stained for FJX1 (i, green) and MYC (ii, red) or FJX1 (iv, green) and the Golgi marker, GM130 (v, red). Nuclei were stained with 4',6-diamidino-2-phenylindole (blue). Respective merged images are shown (iii, vi). Scale bar = 100 μm
Cells were fixed with 4% paraformaldehyde, blocked with 3% BSA and incubated with primary antibody and an Alexa Fluor® was used as the secondary antibody.
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