PE Anti-FITC抗体(ab25539)
Key features and details
- PE Sheep polyclonal to FITC
- Suitable for: WB, ELISA, ICC/IF, Dot blot
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
概述
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产品名称
PE Anti-FITC抗体
参阅全部 FITC 一抗 -
描述
PE羊多克隆抗体to FITC荧光 -
宿主
Sheep -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: WB, ELISA, ICC/IF, Dot blotmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule: Fluorescein Isothiocyanate (Isomer I).
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常规说明
Ab25539 shows cross absorption with mouse, human and rat serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Ab25539 is purified by affinity chromatography. -
Primary antibody说明
Ab25539 shows cross absorption with mouse, human and rat serum proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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ELISA |
Use at an assay dependent dilution.
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ICC/IF |
Use µg for 10 cells.
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Dot blot |
Use at an assay dependent dilution.
|
说明 |
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WB
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
ICC/IF
Use µg for 10 cells. |
Dot blot
Use at an assay dependent dilution. |
靶标
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相关性
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. -
别名
- Fluorescein Isothiocyanate antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab25539 被引用在 2 文献中.
- Zhang D et al. Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathways. Inflamm Res 69:267-278 (2020). PubMed: 31925528
- Ma X et al. Fraxin Alleviates LPS-Induced ARDS by Downregulating Inflammatory Responses and Oxidative Damages and Reducing Pulmonary Vascular Permeability. Inflammation 42:1901-1912 (2019). PubMed: 31273573