Anti-FITC抗体(Phycoerythrin) (ab25539)

概述

  • 产品名称
    Anti-FITC抗体(Phycoerythrin)
    参阅全部 FITC 一抗
  • 描述
    羊多克隆抗体to FITC (Phycoerythrin)
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性
    Ab25539 recognises FITC.
  • 经测试应用
    适用于: WB, ELISA, ICC/IF, Dot blotmore details
  • 免疫原

    Chemical/ Small Molecule: Fluorescein Isothiocyanate (Isomer I).

  • 常规说明


    Ab25539 shows cross absorption with mouse, human and rat serum proteins.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    Ab25539 is purified by affinity chromatography.
  • Primary antibody说明
    Ab25539 shows cross absorption with mouse, human and rat serum proteins.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use µg for 10 cells.
Dot blot Use at an assay dependent dilution.

靶标

  • 相关性
    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • 别名
    • Fluorescein Isothiocyanate antibody

文献

ab25539 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. Yes, this antibody would be suitable for that purpose and has been tested for ICC/IF.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us and for presenting your questions so clearly! To amplify the the signal from the FITC-conjugated anti-Legionella antibody, your idea of using an Alexa Fluor 488-conjugated anti-FITC is a good one. We unfortunately do not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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