概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000 - 1/30000. Suggested coating dilutions: 1/100 - 1/100.
ICC 1/200 - 1/2000.
WB Use at an assay dependent dilution. Colorimetric detection: 1/1000 - 1/10000, Chemiluminescent detection: 1/1000 - 1/30000.
IP Use at an assay dependent dilution. PubMed: 17052454

靶标

  • 相关性FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
  • 别名
    • Fluorescein Isothiocyanate antibody

Anti-FITC antibody (ab19224)参考文献

This product has been referenced in:
  • Zhang M  et al. Expression levels of seprase/FAPa and DPPIV/CD26 in epithelial ovarian carcinoma. Oncol Lett 10:34-42 (2015). Human . Read more (PubMed: 26170973) »
  • Takada K  et al. Targeted disruption of the BCL9/ß-catenin complex inhibits oncogenic Wnt signaling. Sci Transl Med 4:148ra117 (2012). WB . Read more (PubMed: 22914623) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

The antibodies are from different animals and are manufactured in different laboratories so we can expect some variation. The product ab19224 is against FITC which is a derivative of Flouroscein.

I hope th...

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Thank you very much for your interest in ab19224. To our knowledge, ab19224 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab19224 now, test it inIHC-Fr and submit feedback to us in the form of an Abre...

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Thank you for your enquiry. Ab19224 reacts specifically with FITC and there is no reaction with KLH. The antibody has been characterized for application in Western blot, ELISA, and Immunocytochemistry. We don't have any images available at this time, b...

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Thank you for your enquiry. It appears there was an error on the datasheet. This antibody has not been tested in IHC. I have updated the datasheet to reflect this, and I apologize for any inconvenience this may have caused. I hope this informat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"