概述

  • 产品名称
  • 描述
    山羊多克隆抗体to FISH
  • 宿主
    Goat
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human FISH aa 1061-1074 (internal sequence) (Cysteine residue).
    Sequence:

    C-KPIEKSQFIHNNLK


    Database link: Q5TCZ1

  • 阳性对照
    • IHC-P: Human liver tissue.

性能

应用

Our Abpromise guarantee covers the use of ab223516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide.
  • 组织特异性
    Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.
  • 序列相似性
    Belongs to the SH3PXD2 family.
    Contains 1 PX (phox homology) domain.
    Contains 5 SH3 domains.
  • 结构域
    The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-biphosphate (PtdIns(5)P), and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain.
    The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19.
  • 翻译后修饰
    Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Cell projection > podosome. Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adapter protein TKS5 antibody
    • Five SH3 domain-containing protein antibody
    • SH3 and PX domain-containing protein 2A antibody
    • SH3 multiple domains protein 1 antibody
    • Sh3md1 antibody
    • Sh3pxd2a antibody
    • SPD2A_HUMAN antibody
    • TKs5 antibody
    • Tyrosine kinase substrate with five SH3 domains antibody
    see all

图片

  • Paraffin-embedded human liver tissue stained for FISH using ab223516 at 3.8 µg/ml in immunohistochemical analysis, followed by AP-staining.

文献

ab223516 has not yet been referenced specifically in any publications.

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