Anti-Firefly Luciferase抗体[mAb21] (ab64564)

概述

  • 产品名称
    Anti-Firefly Luciferase抗体[mAb21]
    参阅全部 Firefly Luciferase 一抗
  • 描述
    小鼠单克隆抗体[mAb21] to Firefly Luciferase
  • 特异性
    The clone number has been updated from (3i1) to (mAb21) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: WB, ELISA, ICCmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Firefly
  • 免疫原

    Full-length recombinant luciferase from Photinus pyralis.

  • 阳性对照
    • 3T3 cell lysate containing 5ng of recombinant luciferase

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 0.1M Tris glycine, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    mAb21
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    Is unsuitable for IHC-P.
    WB: Use at a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 66 kDa.
    Note: 0.5-2 µg/ml detects 5ng of recombinant luciferase that was added to 3T3 cell lysate.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
    • 细胞定位
      Peroxisome
    • 数据库链接
    • 别名
      • ec 1.13.12.7 antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    Anti-Firefly Luciferase antibody [mAb21] 图像

    • All lanes : Anti-Firefly Luciferase antibody [mAb21] (ab64564) at 5 µg/ml

      Lane 1 : Non-transfected 293 whole cell lysate
      Lane 2 : Firefly Luciferase transfected 293 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : NIH3T3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 66 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

      Lanes 1 - 4: Merged signal (red and green). Green – ab64564 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.

       

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab64564 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    Anti-Firefly Luciferase antibody [mAb21] (ab64564)参考文献

    ab64564 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Loading amount
    0.05 µg
    Gel Running Conditions
    Reduced Denaturing (4-15% Tris-glycine)
    Sample
    Firefly (Photinus pyralis) Recombinant protein (QuantiLum Recombinant Luciferase (Promega))
    Specification
    QuantiLum Recombinant Luciferase (Promega)
    Treatment
    Heated in the presence of sHSP followed by UV crosslinking
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. Rachel Loh

    Verified customer

    提交于 Jan 13 2015

    We have no firefly luciferase antibody tested in IP which is mouse or rabbit origin. Only ab498- Goat polyclonal to firefly Luciferase Other Firefly Luciferase antibodies tested in WB: 1. ab16466 Firefly Luciferase antibody [Luci17] Mouse monocl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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