Anti-Firefly Luciferase抗体[Luci17] (ab16466)

概述

  • 产品名称Anti-Firefly Luciferase抗体[Luci17]
    参阅全部 Firefly Luciferase 一抗
  • 描述
    小鼠单克隆抗体[Luci17] to Firefly Luciferase
  • 经测试应用适用于: Flow Cyt, WB, IHC-Frmore details
  • 免疫原

    Hybridoma produced by the fusion of spleenocytes from mice immunized with luciferase protein isolated from Photinus pyralis.

  • 阳性对照
    • WB: Purified luciferase protein. IHC: frozen drosophila embryos
  • 常规说明

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.08% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯化说明Purified by protein A/G column.
  • 克隆单克隆
  • 克隆编号Luci17
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 18612415ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • 细胞定位Peroxisome
  • 别名
    • ec 1.13.12.7 antibody
    • Firefly antibody
    • Luciferase antibody
    • Luciferin 4 monooxygenase antibody

Anti-Firefly Luciferase antibody [Luci17] 图像

  • Flow cytometric analysis of HEK293 cells expressing Firefly Luciferase, staining Firefly Luciferase with ab16466.

    Cells were fixed with paraformaldehyde at room temperature for 20 min, rinsed twice with permeabilization buffer (PBS, 1% FBS, 0.1% saponin), and incubated with primary antibody. A FITC-conjugated anti mouse IgG was used as the secondary antibody.

Anti-Firefly Luciferase antibody [Luci17] (ab16466)参考文献

This product has been referenced in:
  • Kao T  et al. GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives. Stem Cell Reports 7:518-26 (2016). WB, Flow Cyt . Read more (PubMed: 27594589) »
  • Li J  et al. Real-time detection of CTL function reveals distinct patterns of caspase activation mediated by Fas versus granzyme B. J Immunol 193:519-28 (2014). Read more (PubMed: 24928990) »

See all 10 Publications for this product

Product Wall

The flow cytometry image shown on ab16466's datasheet is from: Pellegatti P et al. Increased level of extracellular ATP at tumor sites: in vivo imaging with plasma membrane luciferase. PLoS ONE 3:e2599 (2008) as stated in the figure legend.

F...

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Thank you for the clarification. EIther one of these antibodies should work well with your sequence. I personally would select ab16466 because it is a purified antibody rather than an IgG fraction, and therefore will have less chance of non-specific bi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Schwann Cell)
Loading amount 200 µg
Specification Schwann Cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Oct 16 2012

Thank you for your enquiry.


Usually the buffer to use for dilutionis the same as your blocking buffer. There are some common buffers that are used which are1-3% BSA buffer, secondary antibody host species serum, Blockase, or even just P...

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Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding these antibodies.

Unfortunately, the sensitivities of these two antibodies has not been determined at this time....

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Thank you for contacting Abcam

We have a western blot image, which is attached showing the antibody with recombinant luciferase. We have not done further testing to see what is either smallest concentration of antibody that is needed to recogn...

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I hope youmanaged to open the attachment file I sent previously. The image contains the list of non-conjugated HRPanti-Firefly antibodies we have in our catalogue, including goat polyclonal, chicken polyclonal, and rabbit polyclonal antibodies. These a...

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Thank you for your enquiry.



We do have a few Firefly antibodies in our catalogue (see attached file)but unfortunately, none of them have been tested in IP application. These products however can be used in WBand might also work in ...

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Thank you for your enquiry and your interest in our products.

Luciferase from the firefly and the luciferase genes can be synthesized and inserted into organisms or transfected into cells. Normally, monkeys do not express luciferase unless th...

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Thank you for contacting Abcam.

This product was created using the whole purified protein and an immunogen. The fusion of spleenocytes is an antibody production technique for creating monoclonals.

The epitope for this antibody has...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"