Anti-Firefly Luciferase抗体(HRP) (ab635)

概述

  • 产品名称
    Anti-Firefly Luciferase抗体(HRP)
    参阅全部 Firefly Luciferase 一抗
  • 描述
    山羊多克隆抗体to Firefly Luciferase (HRP)
  • 偶联物
    HRP
  • 经测试应用
    适用于: Dot blot, ELISA, WBmore details
  • 种属反应性
    与反应: Firefly
  • 免疫原

    Luciferase [Photinus pyralis (Firefly)].

  • 阳性对照
    • Assayed against 1.0µg of firefly luciferase in a standard capture ELISA.
  • 常规说明

    Conjugated to horseradish peroxidase (HRP). Conjugation reference: Farr & Nakane, J.Immunol.Methods 47; 129-144. (1981)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent dilution.
ELISA 1/50000 - 1/100000.
WB 1/10000. See Abreview.

靶标

  • 相关性
    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • 细胞定位
    Peroxisome
  • 数据库链接
  • 别名
    • ec 1.13.12.7 antibody
    • Firefly antibody
    • Luciferase antibody
    • Luciferin 4 monooxygenase antibody

实验方案

文献

This product has been referenced in:
  • MacKay JA  et al. HIV TAT peptide modifies the distribution of DNA nanolipoparticles following convection-enhanced delivery. Mol Ther 16:893-900 (2008). Read more (PubMed: 18388927) »
  • Nasim MT & Eperon IC A double-reporter splicing assay for determining splicing efficiency in mammalian cells. Nat Protoc 1:1022-8 (2006). Goat . Read more (PubMed: 17406339) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your enquiry. We only have the product sizes available as they are noted on our online datasheets. We typically do not offer small sample sizes. We do guarantee our antibodies based on the tested applications and species reactivities. For...

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Thank you very much for your enquiry. Unfortunately, I have not been able to obtain any positive data for either formalin or frozen samples from our laboratory. As such, I feel that we should not be marketing this for use in IHC or IM and I have...

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I'm sorry to hear you are experiencing problems with ab635. We have had very few complaints about ab635 and none with the lot you are using. I understand this is a replacement vial after you experienced problems with ab3279 and after trying my proto...

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Further to our telephone conversation this morning I have been in correspondence with the source of this antibody. They confirmed with me that this antibody is yet to be tested using IHC-Fr. They could not tell me where the information regarding the di...

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Thank you for sending us your detailed protocol. We are very sorry to hear that you are having problem with this antibody. We would like to draw your attention to the fact that this antibody has only been tested and characterized for IHC on frozen ...

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No, this antibody has not been tested in a Sandwich ELISA, nor have any of our other antibodies to fire fly luciferase. However, we recommend that you use the mouse monoclonal ab7358 on the bottoem (i.e. as a coating antibody)and a conjugated version o...

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ab635 has not been tested for this purpose. A potential problem is that some fixation techniques destroy the epitopes to which the antibodies bind. I believe that this technique has been shown to work with a similar antibody but I do not have the refer...

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This antibody can be used for direct detection of luciferose in Western blots using any suitable HRP substrate e.g. ECL.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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