Anti-Firefly Luciferase抗体(ab99944)

概述

  • 产品名称
    Anti-Firefly Luciferase抗体
    参阅全部 Firefly Luciferase 一抗
  • 描述
    兔多克隆抗体to Firefly Luciferase
  • 特异性
    This antibody recognizes firefly luciferase.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Other
  • 免疫原

    Recombinant Chinese Firefly Luciferase

  • 阳性对照
    • Firefly luciferease

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, 5mM EDTA, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 61 kDa.

靶标

  • 相关性
    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
  • 细胞定位
    Peroxisome
  • 别名
    • ec 1.13.12.7 antibody
    • Firefly antibody
    • Luciferase antibody
    • Luciferin 4 monooxygenase antibody

图片

  • All lanes : Anti-Firefly Luciferase antibody (ab99944) at 1 µg/ml

    Lane 1 : Firefly luciferase at 0.02 µg
    Lane 2 : Firefly luciferase at 0.05 µg


    Predicted band size : 61 kDa

实验方案

文献

ab99944 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your email and providing the required data.

I could replace the antibody at no cost however as we do not have received complaints about this lot so I am wondering the problem might be either with the expression of luciferase or w...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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I hope youmanaged to open the attachment file I sent previously. The image contains the list of non-conjugated HRPanti-Firefly antibodies we have in our catalogue, including goat polyclonal, chicken polyclonal, and rabbit polyclonal antibodies. These a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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